绿色荧光蛋白基因在牦牛乳腺上皮细胞中表达的研究

Study on expression of green fluorescent protein in yak mammary epithelial cells

从牦牛乳腺采集组织,并通过胶原酶消化法和胰蛋白酶消化法相结合在体外成功分离、纯化到了牦牛乳腺上皮细胞.通过观察,具有明显的上皮细胞特征,而且符合一般细胞的生长规律.通过脂质体介导法将携带有绿色荧光蛋白的外源基因转染进乳腺上皮细胞中,在荧光显微镜下检测到了绿色荧光蛋白基因的表达.

Purified yak mammary epithelial cells are isolated by using collagenase digestion of the mammary gland tissue for primary cell culture, and purifying them with time-controlled trypsinization. Observation reveals that the cells possess typical character of epithelial cells. Moreover, the cells coincide general law of cell growth. The gene EGFP is successfully transfected into yak mammary epithelial cells using liposomes transfection, and the expressing of EGFP under fluoroscope is observed.