金翁止痢颗粒对免疫低下小鼠的免疫调节作用

The Effects of the Jin Weng Zhi Li Granule on the Immune Regulation in Immunodepressed Mice

为探讨金翁止痢颗粒对免疫低下小鼠的免疫调节作用,将72只小鼠随机分为空白组、模型组、阳性药物组、金翁止痢颗粒高剂量组、中剂量组、低剂量组,其中空白组小鼠给予生理盐水,模型组与各药物组小鼠腹腔注射环磷酰胺构建免疫低下模型.造模成功后,模型组给予生理盐水,阳性药物组给予2g/kg黄芪多糖液,金翁止痢颗粒高剂量组、中剂量组、低剂量组分别灌胃20g/kg,16g/kg,12.8g/kg的金翁止痢颗粒提取液,1次/d,连续治疗7d后,检测小鼠碳粒廓清指数、免疫器官指数,T/B淋巴细胞转化等指标.结果表明,金翁止痢颗粒能显著提高免疫低下小鼠碳粒廓清指数、免疫器官指数,促进T/B淋巴细胞转化,与模型组相比差异极具有统计学意义(p<0.01),表明金翁止痢颗粒具有增强免疫低下小鼠免疫功能的作用.

This study was designed to explore the effect of the Jin Weng Zhi Li granule on immune regulation of immunosuppressed mice.72 mice were divided randomly into blank group which was given physiological saline,the high dose,middle dose,low dose group of Jin Weng Zhi Li granule,model group and positive medicine group were used to establishe the immunosuppressed mice models by abdominal injection of cyclophosphamide.After successful modeling,model group was given physiological saline,positive medicine group was given 2g/kg Astragalus polysaccharides.20g/kg,16g/kg,12.8g/kg of the Jin Weng Zhi Li granule extract were given to the high dose,middle dose,low dose group of Jin Weng Zhi Li granule with once a day for 7days by gavage.The carbon clearance index,immune organ index,T and B lymphocyte proliferation in immunosuppressed mice were measured.The results showed that Jin Weng Zhi Li granule could increase the carbon clearance index,immune organ index and T,B lymphocyte proliferation in immunosuppressed mice,compared with model group,the difference was extremely significant(p<0.01).It suggested that Jin Weng Zhi Li granule could enhance immune function of immunosuppressed mice.