赤芍、栀子、大黄修复大鼠CCI_4肝损伤的细胞病理学研究续报

Cycomorphological,Cytochemical Study on CCI_4-induced Liver Necrosis in Rats Treated with-induced Liver Necrosis in Rats Treated with Pr,FG or Rp:A Continuation Report

将43只实验大鼠分成5组:肝损伤组给CCI_4皮下注射;大黄组、赤芍组、栀子组除给CCI_4皮下注射外,分别给以上述各药煎剂灌胃各共7日;对照组皮下注射生理盐水,并以自来水灌胃。结果,三个中药治疗组与肝损伤组相比,除栀子组外,大黄组及赤芍组均使肝损伤区域缩小,肝细胞中脂滴减少,细胞核增大,RNA、核仁增多,糖原恢复,琥珀酸脱氢酶和葡萄糖-6-磷酸酶活性增强,粗面内质网恢复平行排列,滑面内质同增加,线粒体形态结构恢复、数量增加,表明大黄及赤芍对CCI_4所致肝损伤均有修复作用(大黄更佳),其机制可能是通过内质同损伤的阻止,促进蛋白质合成及解毒,恢复了肝功能。

In TCM-WM therapy of severe viral hepatitis,certain therapeutic effects had been shown by some decoctions,i.e.a reinforced decoct.of compo- site prescription Herba Artemisiae scopariae,decoct.Radix et Rhizoma Rhei,cooling blood & clearing heat with heavy Radix Paeoniae rubra, as well as a composite prescription-decoct.of Pr-FG-Rp（P.r.,Fructus Gardenia jasminaides,and Rheum palmatum）.In this present experiment, as a continuation study following the authors’rat experiment with comp- osite prescription Pr-FG-Rp（Shu,et al,1988）,repairing effects of de- coctions Pr,FG and Rp were compared with each other in 28 CCI4-induced liver necrosis Wistar rats,in order to provide further cytomorphological basis for clinical trials and probing into their pharmacodyamics. Altogether 43 experimental Wistar rats were divided into five groups. The liver damage group（10 rats）was poisoned via hypodermal injectioils （hdi） with CCI4;as for the three treatment groups,besides CCI4 hdi, Pr,FG or Rp was administrated daily through gastric intubation（gt） for 7 days;while the control group（5 rats）was administrated with normal saline hdi and tap water gt only.Liver histopathological and ultrastructure speci mens following decapitation of the animals at the 8th day showed that,of the three treated groups,besides the still more or less severe damages in group FG,the other two groups both displayed only mild damages,in hepatic lobule,the necrotic area and the liver cellul- arfat droplets decreased,while RNA,glycogen increased,nucleole and SDH- ase,G-6-Pase activities increased as well,and the roughendopl asmic reti- culumrecovered to normal range,the mitochondria regained the normal str- ucture and increased in number.It is suggested that both Rp and Pr are able to prevent liver damage,and Rp better than Pr.Their mechanism mi- ght be that they prevent and repair the damage of endoplasmic reticulum, promoting protein synthesis and detoxication,thus restore and recover the liver functions.