白藜芦醇对生长育肥猪抗氧化能力、空肠黏膜免疫及结肠菌群的影响

Effects of Resveratrol on Antioxidant Capacity,Jejunal Mucosal Immunity and Colonic Microflora of Growing-Finishing Pigs

本试验旨在研究饲粮中添加白藜芦醇对生长育肥猪抗氧化能力、空肠黏膜免疫及结肠菌群的影响。试验选取体重[(24.67±3.49) kg]相近的"杜×长×大"生长育肥猪36头,随机分为2个组,分别采食基础饲粮和含白藜芦醇饲粮(基础饲粮+600 mg/kg白藜芦醇),每组6个重复,每个重复3头猪。试验期共119 d。试验结束时,每个重复随机选择1头体重接近平均体重的试验猪采集血清、肝脏、空肠黏膜和结肠食糜样品,保存待测。结果表明:1)与基础饲粮组相比,饲粮中添加白藜芦醇对试验猪生长育肥全程生长性能无显著影响(P>0.05); 2)与基础饲粮组相比,饲粮中添加白藜芦醇显著提高试验猪血清中过氧化氢酶(CAT)活性以及总抗氧化能力(T-AOC)(P<0.05),但对空肠黏膜和肝脏中抗氧化指标无显著影响(P>0.05); 3)与基础饲粮组相比,饲粮中添加白藜芦醇显著降低试验猪空肠黏膜白细胞介素-6(IL-6)含量(P<0.05),显著提高空肠黏膜白细胞介素-10(IL-10)含量(P<0.05); 4)与基础饲粮组相比,饲粮中添加白藜芦醇显著提高试验猪结肠食糜中双歧杆菌数量(P<0.05),有降低大肠杆菌数量的趋势(P=0.05)。由此得出,生长育肥全期,饲粮中添加白藜芦醇可调节生长育肥猪结肠菌群,增强机体抗氧化与空肠黏膜抗炎的能力,改善肠道健康。

This study aimed to evaluate the effects of dietary resveratrol on antioxidant capacity,jejunal mucosal immunity and colonic microflora of growing-finishing pigs.A total of 36 Duroc×Landrace×Yorkshire growingfinishing pigs at a body weight of(24.67±3.49) kg were divided into 2 groups with 6 replicates per group and 3 pigs per replicate,and pigs were fed a basal diet or a resveratrol diet(basal diet+600 mg/kg resveratrol).The whole experiment lasted for 119 days.At end of the experiment,one pig per replicate close to the average weight was chosen to slaughter and sample blood,liver,jejunum mucosa and colonic content.The results showed as follows:1) compared with the basal diet group,dietary resveratrol supplementation had no significant effect on growth performance of growing-finishing pigs(P>0.05) in the whole experiment period.2) Compared with the basal diet group,dietary resveratrol supplementation significantly increased serum total antioxidant capacity(T-AOC) and catalase(CAT) activity(P<0.05).No significant difference in the antioxidant parameters in liver and jejunal mucosa was observed(P>0.05).3) Compared with the basal diet group,the supplementation of resveratrol significantly decreased the content of interleukin-6(IL-6)(P<0.05) and increased the content of interleukin-10(IL-10)(P<0.05) in jejunal mucosa.4) Compared with the basal diet group,resveratrol significantly increased the number of Bifidobacteria(P < 0.05) and tended to reduce the number of Escherichia coli(P=0.05) in colonic content.These results suggest that supplementation of resveratrol in diets can improve colonic flora structure,serum antioxidant capacity and jejunal mucosal anti-inflammatory capacity in growing-finishing pigs.