仔猪断奶后1周内血清生化指标、十二指肠形态结构及空肠中钠/葡萄糖共转运载体1与二肽转运载体1基因表达量的变化

Changes in Serum Biochemical Indices,Duodenal Morphology,Expression Levels of Sodim-Gluose Transporter 1 and Peptidetransporter 1 Genes in Jejunum of Piglets at the First Week Post-Weaning

本试验旨在探讨仔猪在断奶前及断奶后1周内血清生化指标、十二指肠形态结构及空肠中钠/葡萄糖共转运载体1(SGLT1)和二肽转运载体1(PepT1)基因表达量的变化规律。试验选取20日龄仔猪55头,断奶前1天屠宰5头,余下50头仔猪在21日龄断奶,并分为5组(圈),每组10头仔猪,分别在断奶后第2、4和8天从每组各挑选1头仔猪屠宰。结果表明:1)与断奶前相比,血清碱性磷酸酶活性在断奶后第2天显著降低(P<0.05),在断奶后第8天极显著降低(P<0.01),血清钾离子含量在断奶后第4和8天显著增加(P<0.05),血清尿素氮含量在断奶后第2天极显著增加(P<0.01),在断奶后第8天又显著降低(P<0.05),血清免疫球蛋白G含量显著降低(P<0.05)。2)与断奶前相比,十二指肠绒毛高度在断奶后第2天极显著降低(P<0.01),断奶后第4天显著降低(P<0.05),隐窝深度在断奶后第2和4天均显著增加(P<0.05),两者比值在断奶后第2和4天均显著降低(P<0.05)。3)与断奶前相比,空肠中SGLT1基因表达量在断奶后均显著降低(P<0.05),空肠中PepT1基因表达量在断奶后第4和8天均显著降低(P<0.05)。由此表明,断奶1周内对仔猪生理机能造成了一定的应激;断奶降低了仔猪血清免疫球蛋白G含量,改变了仔猪十二指肠形态结构,降低了空肠中SGLT1和PepT1基因表达量,以断奶后第2和4天应激最强。

The experiment was conducted to investigate the changes in serum biochemical indices,duodenal morphology and expression levels of sodim-gluose transporter 1(SGLT1) and peptidetransporter 1(PepT1)genes in jejunum of piglets at pre-weaning and the first week post-weaning.A total of 55 piglets at 20 days of age were selected,five piglets were slaughtered at 1 d pre-weaning,and the remained 50 piglets(weaned at 21 days of age) were randomly divided to 5 groups(pens) with 10 piglets per pen.One piglet per pen was sacrificed on d 2,4 and 8 post-weaning,respectively.The results showed as follows:1) compared with pre-weaning,the alkaline phosphatase activity in serum on d 2 post-weaning was significantly decreased(P<0.05) and was very significantly decreased on d 4 post-weaning(P<0.01);serum potassium ion content was significantly increased on d 4 and 8 post-weaning(P<0.05);serum urea nitrogen content was very significantly increased on d 2 post-weaning(P<0.01) and significantly decreased on d 8 post-weaning(P<0.05);serum immunoglobulin G content was significantly decreased after weaning(P<0.05).2) Compared with pre-weaning,the duodenal villus height was significantly decreased on d 2 post-weaning(P<0.01) and d 4 post-weaning(P<0.05),crypt depth in duodenum was significantly enhanced on d 2 and 4 post-weaning(P<0.05),and the ratio of villus height to crypt depth was also significantly decreased on d 2 and 4 post-weaning(P<0.05).3)Compared with pre-weaning,the expression levels of SGLT1 gene in jejunum was also significantly decreased after weaning(P<0.05);the expression level of PepT1 gene in jejunum on d 4 and 8 post-weaning was significantly decreased(P<0.05).These results suggest that weaning on the first week causes a certain stress on physiological functions of piglets.Weaning reduces the serum immunoglobulin G content,changes the duodenal morphology,decreases the expression levels of SGLT1 and PepT1 genes in jejunum,which may affect the digestion and absorption of carbohydrates and proteins,especially on d 2 and 4 post-weaning.