混合真菌发酵对玉米秸秆纤维素与木质素降解率的影响

Effects of Mixed Fungal Fermentation on Degradation Rate of Cellulose and Lignin of Corn Straw

本试验以拟康宁木霉(Trichoderma koningiopsis)和黄孢原毛平革菌(Phanerochaetechrysosporium)为发酵菌种,研究混合发酵对玉米秸秆纤维素和木质素降解率的影响及最优发酵条件。采用单因素试验设计,通过测定菌种比例(拟康宁木霉∶黄孢原毛平革菌)、接种量、营养液添加量及发酵天数对纤维素和木质素降解率的影响,筛选出混合真菌发酵的最优条件,并进一步采用响应面法评价各因素及其交互作用对玉米秸秆纤维素和木质素降解率的影响,最后采用Design Expert 8.0.6软件分别建立混合真菌发酵玉米秸秆降解纤维素和木质素条件的二次多项式数学模型。结果表明:混合真菌发酵玉米秸秆的最优发酵条件为菌种比例1.0∶4.1,接种量25.2%,营养液添加量72.2%,发酵天数11 d。在此条件下,玉米秸秆纤维素和木质素降解率分别达到36.80%和28.87%。

In this experiment,Trichoderma koningiopsis and Phanerochaete chrysosporium were used as fermentation strains,the effects of mixed fungal fermentation on degradation rate of cellulose and lignin of corn strawand the optimal fermentation parameters were investigated. Single factor experiment was used to study the effects of the fungus ratio (Trichoderma koningiopsis:Phanerochaete chrysosporium),inoculation amount,additive amount of nutrient solution and fermentation day on the degradation rate of cellulose and lignin. On the basis of this experiment,the optimal fermentation factors were selected,and the effects of various factors and their interactions on the degradation rates of cellulose and lignin were further evaluated by response surface method. The quadratic polynomial mathematical model of degradation rates of cellulose and lignin of corn strawby mixed fungus fermentation was established by using design expert 8.0.6 software. The results showthat the optimal parameters of mixed fungus fermentation of corn straware as follows: fungus ratio 1.0:4.1,inoculation amount 25.2%,additive amount of nutrient solution 72. 2%,fermentation days 11 days. On this condition,the degradation rates of cellulose and lignin reach 36.80% and 28.87%,respectively.