摘要
采用半定量 ＲＴ－ＰＣＲ法测定了１，６，１２月龄 Ｃ５７小鼠海马βＮＧＦ ｍＲＮＡ水平，将β肌动蛋白基因作为内标。结果表明， ６， １２月龄两组间无显著性差异，但均显著低于１月龄组，说明Ｃ５７小鼠发育过程中其海马 βＮＧＦ基因转录水平明显降低。另外，发现Ｃ５７小鼠海马 β ＮＧＦ基因第２９４位碱基为Ｃ，与普通小鼠的Ｔ不同，但这种碱基差异并不影响氨基酸组成。
β-Nerve growth factor (β NGF) mRNA levels in hippocampa from C57BL/6J mice of different ages were compared by semi-quantitative RT-PCR method, taking β-actin gene as an internal control. The levels of 6-and 12-month-old C57 mice were much lower compared with that of the l-month-old mouse. However, there was no significant difference between these two groups. This result suggests that gene transcription level of hippocampus β NGF in a C57, mouse descends evidently as it grows up. Base T at Site 294 of β NGF cDNA was substituted by C in the C57, mouse, as revealed by DNA sequencing for the first time. Nevertheless, this polymorphism of nucleotide did not make any difference in amino acid composition.
