Temperature Effects on Seed Dormancy Relieving of Fritillaria thunbergii Miq

浙贝母种子的休眠在８～１０℃下需要５０天时间能够解除，而在３～５℃下则需要８０天时间。为了研究浙贝母种子休眠解除的生理基础，利用分光光度法和电泳方法研究了过氧化物酶、酸性磷酸酶和蛋白质的变化。结果表明，随着低温处理，过氧化物酶活性和酸性磷酸酶的活性升高，蛋白质电泳图谱上有新带出现。这些变化最终导致了休眠的解除。

The seed dormancy relieving of Fritillaria thunbergii Miq required 50 days at 8～10℃and 80 days at 3～5℃ In order to study the physiological basis for seed dormancy relieving,peroxidase,acid phosphatase and proteins were assayed by spectrophotometric analysis and electrophoresis The results showed that peroxidase activity and acid phosphatase activity increased and new bands were detected in protein pattems as the seeds were treated with low temperature,these changes resulted in the seed dormancy relieving