流式细胞术检测免疫表型和分析DNA倍体对非霍奇金淋巴瘤患者的临床价值

Clinical Value of Immunophenotype Detection and DNA Ploid Analysis by Flow Cytometry for Non-Hodgkin's Lymphoma Patients

目的:探讨流式细胞术免疫表型检测及DNA倍体分析对于非霍奇金淋巴瘤(NHL)患者的临床价值。方法:收集2007年8月至2015年3月我院收治的NHL患者80例为研究对象,作为观察组。选取同时期收治的20例反应性淋巴结增生患者为对照组。应用流式细胞术检测免疫表型和分析DNA倍体。结果:观察组患者DNA异倍体检出率、DAN指数(DI)值和SPF均高于对照组,差异有统计学意义(P<0.05)。NHL-Ⅰ、NHL-Ⅱ患者SPF、DI值与对照组比较,差异无统计学意义(P>0.05);NHL-Ⅲ和NHL-Ⅳ患者的SPF、DI值与对照组比较,差异有统计学意义(P<0.05)。低度恶性组、中高恶性组患者SPF和DI值与对照组比较,差异有统计学意义(P<0.05);中高恶性组患者SPF和DI值与低度恶性组比较,差异有统计学意义(P<0.05)。结论:流式细胞术免疫表型检测并进行DNA倍体分析,通过DI和SPF水平,能够反映肿瘤增殖情况和患者病情的恶化程度、指导预后。

Objective:To explore the clinical values of detecting immunophenotype and analyzing DNA ploid by flow cytometry for patients with non-Hogkin's lymphoma(NHL).Methods:Eighty NHL patients admitted in our hospital from August 2007 years to March 2015 Years were included in the observation group,20 patients with reactive lymphoid hyperplasias were selected as control group.The immunophenotypes were detected and the DNA ploid was analyzed by flow cytometry.Results:The detected rate of DNA aneuploidy,DAN index(DI) and SPF in observation group were higher than those in control group,there was signifificant difference(P <0.05).The SPF and DI in patients with NHL-Ⅰ,NHL-Ⅱ had no statistical difference as compared with that in control group(P > 0.05);but the SPF and DI in pateints with NHL-Ⅲ and patients with NHL-Ⅳ showed statistical significance as compared with that in control group(P < 0.05).The SPF and DI in patients with low malignancy group and middle malignancy group showed statistical significance as compared with control group(P < 0.05).The SPF and DI in middle malignancy group had statistical significance as compared with that in low malignancy group(P < 0.05).Conclusion:the immunophenotype detection and DNA ploid analysis by flow cytometry can reflect the tumor proliferation and deterioration of patients with Non-Hogkin's lymphoma,predicting the prognosis.