CCR7在成人急性白血病中的表达及其临床意义

Expression and Clinical Significance of CC-chemokine Receptor 7 in Adult Acute Leukemia

目的:探讨成人急性白血病中的趋化因子受体-7(CC-chemokine receptor 7 CCR7)表达及其与临床特征的关系。方法:应用流式细胞术检测71例成人急性白血病患者(年龄≥12岁)骨髓白血病细胞CCR7的表达水平,探讨其在成人急性白血病中的表达及与年龄、性别、诊断分型、外周血WBC数、骨髓原始幼稚细胞比例、CD56表达、分子生物学、细胞遗传学,危险度分层、髓外浸润等临床特征的关系。结果:19例急性淋巴细胞白血病(ALL)中CCR7^+者7例(36.8%),52例急性髓系白血病(AML)中CCR7^+者5例(9.6%),在ALL中CCR7^+率明显高于AML中(P<0.05),在ALL中CCR7^+组髓外浸润发生率明显高于CCR7-组(100%vs 41.7%)(P<0.05),ALL伴有髓外浸润组CCR7平均荧光强度(MFI)明显高于无髓外浸润组(50.00±10.42 vs 18.14±1.39)(P<0.05)。结论:CCR7在成人急性白血病中表达增加,在ALL中阳性率高于AML中,并与ALL髓外浸润相关。

Objective:To explore the expression of CC-chemokine Receptor 7(CCR7) in adult acute leukemia patients,and to analyze the relationship of CCR7 expression with the clinical characteristics of patients.Methods:The expression of CCR7 in bone marrow samples from adult acute leukemia patients were detected by flow cytometry(FCM),the relationship of CCR7 expression with the clinical characteristics of patients such as sex,age,WBC count,blast cell ratio,CD56 expression,molecular biology,cell genetics,risk stratification,extramedullary infiltration was analyzed.Results:The expression rate of CCR7 in adult ALL and AML patients was 36.8%and 9.6%,respectively,and the expression level of CCR7 in ALL patients was higher than that in AML patients(P <0.05).The extramedullary infiltration rate was 100%and 41.7%for CCR7 positive and negative groups of ALL,respectively(P <0.05).While the mean fluorescence intensity(MFI) in extramedullary infiltration group of ALL was higher than that in noneextramedullary infiltration group of ALL(50.00 ± 10.42 vs 18.14 ± 1.39),respectively(P < 0.05).Conclusion:CCR7 is higher expressed in adult acute leukemia cells,moreover its expression rate in ALL is higher than that in AML,and the expression of CCR7 is related with extramedullary infiltration in ALL.