CIK细胞输入治疗对老年多发性骨髓瘤患者外周血免疫细胞水平影响及安全性分析

Effect and Safety of CIK Cell Infusion on Peripheral Blood Immune Cell Level in the Elderly Patients with Multiple Myeloma

目的:探究CIK细胞输入治疗对老年多发性骨髓瘤患者外周血免疫细胞水平的影响及安全性。方法:将2004年4月至2015年4月来本院初诊为多发性骨髓瘤的60例患者随机分为对照组和观察组。对照组给予VAD化疗,观察组在对照组的基础上进行CIK细胞输入治疗。利用ELISA试剂盒测定血清中IL-17、IL-6和转化生长因子(TGF)-β含量;检测血红蛋白,血沉和血肌酐水平;观察患者不良反应发生情况;疗程结束后对两组患者进行疗效判定。结果:治疗前,两组患者的IL-17,IL-6和TGF-β水平无差异(P>0.05)。治疗后,两组患者的IL-17、IL-6和TGF-β水平均降低,但观察组水平明显低于同期的对照组(P<0.05)。治疗前两组患者的CD3^+CD4^+,CD3^+CD8^+和CD3^+CD4^+/CD3^+CD8^+水平无差异(P>0.05),治疗后均降低,且观察组水平明显低于同期的对照组,(P<0.05)。患者发生恶心呕吐、心悸胸闷,心肌酶升高,转氨酶升高和肌酐增高等副反应的例数两组间无差异。观察组临床有效率显著高于对照组(P<0.05)。结论:CIK细胞输入治疗老年多发性骨髓瘤具有良好的临床效果,其机制是通过降低免疫抑制因子的水平显著增加外周血免疫细胞数。

Objective:To explore the effect of CIK cells on the level of peripheral blood immune cells in the elderly patients with multiple myeloma and its safety.Methods:A total of 60 patients with multiple myeloma from April 2004 to April 2015 in our hospital were enrolled in the study.According to the treatment plan,the patients were randomly divided into control and observation group.The patients in control group was given VAD chemotherapy,the patients in observation group was treated with CIK cells on basis of the control group protocol.ELISA was used to detect the serum levels of IL-17,IL-6 and transforming growth factor(TGF);the hemoglobin,erythrocyte sedimentation rate(ESR) and serum creatinine were assayed also.The incidence of adverse reaction in patients was assayed;the therapeutic efficacy of observation and control groups was judged after treatment curses.Results:The serum levels of IL-17,IL-6 and TGF-βbetween two groups before treatment were not significantly different(P >0.05),but after treatment,thier levels in two groups decreased,moreover the levels of the observation group was significantly lower than that in control group(P <0.05).Before treatment,there was no significant difference in the levels of CD3^+ CD4^+,CD3^+ CD8^+ and CD3^+CD4^+ /CD3 ^+ CD8 ^+ between the two groups(P >0.05);after treatment,these levels all decreased,moreover the levels of the observation group significantlly lower than that in control group(P <0.05).The incidence of nausea and vomiting,heart palpitations,chest tightness,increase of myocardial enzyme,amino transferase and creatinine all were not significantly different between two groups(P > 0.05).The curative efficiency of the observation group was significantly higher than that of the control group(P <0.05).Conclusion:CIK cell therapy has better curative effect in the elderly patients with multiple myeloma.The level of peripheral blood immune cells can be significantly increased by decreasing the level of immunosuppressive factor.