细胞遗传学检查在诊断非霍奇金淋巴瘤患者骨髓受累中的应用

Application of Cytogenetic Test for Diagnosis of Bone Marrow Involvement in Patients with Non-Hodgkin's Lymphoma

目的:分析细胞遗传学检查在诊断非霍奇金淋巴瘤骨髓受累中的作用。方法:总结74例初治非霍奇金淋巴瘤患者骨髓标本的形态学、细胞遗传学、流式细胞术和分子生物学的检测结果,比较单一检测和联合检测的异常检出率。结果:骨髓形态学、细胞遗传学、流式细胞学和分子生物学的异常检出率分别为21.6%、17.6%、23.0%和33.8%,4种方法联合检测可将检出率提高至44.6%。在诊断非霍奇金淋巴瘤骨髓受累方面,细胞遗传学、骨髓形态学、流式细胞学和分子生物学的检测结果一致,无差异。结论:虽然细胞遗传学的检出率较低,但是在一些患者中可以检测出其它方法未检测到的异常。联合使用4种检测方法可以提高骨髓受累的检出率。

Objective:To investigate the role of cytogenetic analysis in the detection of bone marrow( BM)involvement in patients w ith non-Hodgkin's lymphoma( NHL). Methods: The bone marrow samples of 74 patients w ith NHL w ere detection by using morphology,cytogenetic test,flow cytometry and molecular biological assay. The detected results of morphology,cytogenetic test,flow cytometry and molecular biological assay alone and thier combined detection w ere compared,the detective rate and consistencies of the 4 methods w ere analyzed. Results: The detection rates of BM involvement by using morphology,cytogenetic,flow cytometry,and molecular biological assays w ere 21. 6%,17. 6%,23. 0% and 33. 8% respectively. The detective rate w as enhanced to 44. 6% by combining the 4 methods. Cytogenetic test show ed the result consistent w ith the other methods. Conclusion: Although cytogenetic test show s a low er detective rate than the other methods,but in some patients the cytogenetic test can detect the abnormality of bone marrow w hich can not be detected by other methods alone,the combination test of 4 detection methods can enhance the detectable rate of BM involvement.