动员后骨髓和外周血采集物中T细胞CCR5表达的对比研究

Comparison of CCR5 Expression on T Lymphocytes between the Bone Marrow and Peripheral Blood Grafts after Mobilization

目的:研究动员后骨髓和外周血采集物中T细胞CCR5表达的异同,分析移植物中T细胞CCR5的表达与移植后急性移植物抗宿主病(GVHD)发生的相关性。方法:选取行亲缘异基因造血干细胞移植的46对供受者为研究对象,应用重组人粒细胞集落刺激因子(rhG-CSF)动员后,测定并比较骨髓(G-BM)和外周血采集物(G-PB)中CCR5^+T细胞的相对比例和绝对数量,然后分析输注的CCR5^+T细胞数量和移植后Ⅱ-Ⅳ度急性GVHD发生的相关性。结果:G-PB中CD3^+CD4^+及CD3^+CD8^+T细胞的CCR5^+细胞所占的比例均明显低于G-BM(P<0.01),但G-PB中CD3^+CD4^+CCR5^+及CD3^+CD8^+CCR5^+T细胞的绝对数均是骨髓中的15-25倍,且输注的CCR5^+T细胞的绝对数量不是受者发生急性GVHD的危险因素(P>0.05)。结论:G-PB和G-BM中CCR5^+T细胞比例和绝对数量存在显著差别,不同来源的干细胞具有不同的免疫特性。此外,移植后急性GVHD的发生与移植物中CCR5^+T细胞的数量无关,而可能受其相对比例影响。

Objective: To compare the expression of C-C chemokine receptor type 5( CCR5) on T cells betw een bone marrow grafts( G-BM) and peripheral blood grafts( G-PB) nobilized by recombinant human granulocyte colonystimulating factor( rh G-CSF),and to analyze the correlation of CCR5^+T lymphocyte expression in the grafts w ith the occurrence of acute GVHD. Methods: Forty-six healthy donor and their recipient pairs of related allogeneic hematopoietic stem cell transplantation( allo-HSCT) w ere enrolled in this study. All the recipients w ere received the infusion of GBM and G-PB. The relative proportion and quantity of CCR5^+T cell subset in G-BM and G-PB w ere detected and compared. Then the correlation of the quantity of infused CCR5^+T cells w ith the occurrence of acute GVHD w as analyzed.Results: After mobilization,the proportions of CD4^+CCR5^+and CD8^+CCR5^+T cells occupying T cells in G-PB w ere both low er than those in G-BM. How ever,the absolute counts in G-PB w ere 15- 25 times more than those in the bone marrow. And the absolute counts could not predict the occurrence of acute GVHD after transplantation( P > 0. 05).Conclusion: The difference of CCR5^+subsets betw een G-PB and G-BM may partially explain that grafts from different sources have different immunologic characteristics. Besides,the quantity of CCR5^+T cells in the grafts are not related w ith the occurrence of acute GVHD. How ever,the relative proportion of CCR5^+T cell subset in the grafts may be predictive of acute GVHD.