乙肝患者HBV感染模式与HBV-DNA检测结果分析

Analysis of HBV Infection Pattern and HBV-DNA Quantitative Content in Patients with Hepatitis B

目的分析300例乙肝患者的乙肝两对半与乙肝病毒DNA(HBV-DNA)检测结果。方法化学发光法检测乙肝两对半,荧光定量PCR检测乙肝DNA含量。结果在300例乙肝两对半6组模式中,其中乙肝HBsAg+HBeAg+HBcAb模式中HBV-DNA含量阳性率最高,其中HBsAg+HBeAb、HBsAg+HBcAb和HBsAg+HBsAb+HBeAb+HBcAb这3种模式与HBsAg+HBeAg+HBcAb比较有显著差异(P <0.05)。在111例HBeAg阳性患者中,96.4%HBV-DNA阳性;279例HBeAg阴性患者中,44.4%HBV-DNA阳性。HBV-DNA载量与HBsAg、HBeAg含量的关系中,HBeAg含量增高,HBV-DNA载量也增高,具有一定的相关性。但HBsAg含量与HBV-DNA载量之间并不具有相关性。结论乙肝DNA检测可补充乙肝两对半检测的不足,能更好地反映病毒的复制及感染状态。

Objective To analyze the results of hepatitis B virus and hepatitis B virus DNA(HBV-DNA) in patients with hepatitis B. Methods The chemiluminescence method was used to detect two pairs of hepatitis B in 300 patients. The content of hepatitis B DNA was detected by real-time PCR. Results Among the 300 cases of hepatitis B,the positive rate of HBV-DNA was the highest in hepatitis B HBsAg + HBeAg + HBcAb mode,and the three modes of HBsAg + HBeAb,HBsAg + HBcAb and HBsAg + HBsAb + HBeAb + HBcAb were significantly different from HBsAg + HBeAg + HBc Ab(P <0.05). Of the 111 HBe Ag-positive patients,96.4% were HBV-DNA positive;of the 279 HBe Ag-negative patients,44.4% were HBV-DNA positive. In the relationship between HBV-DNA load and HBs Ag and HBe Ag content,HBeAg content increased,HBV-DNA load also increased,and there was a certain correlation. However,there is no correlation between HBs Ag content and HBV-DNA loading. Conclusion Hepatitis B DNA test can supplement the deficiency of hepatitis B detection,which can better reflect the virus replication and infection status.