二烯丙基二硫对AD模型小鼠学习记忆能力和海马突触的影响

Effect of diallyl disulfide on learning and memory abilities and hippocampal synapses in mouse models of Alzheimer's disease

目的探讨二烯丙基二硫(DADS)对阿尔茨海默病(AD)小鼠学习记忆能力及海马突触的影响。方法侧脑室立体定位术注射凝集态Aβ1-42制备AD模型小鼠,随机分成四组AD模型组,低中高DADS剂量组,分别灌服0、10、50和100 mg/kg/d的DADS。1个月后各组小鼠进行Morris水迷宫检测学习记忆能力、银染和电镜检测海马CA1区树突棘和突触的结构、蛋白印迹和RT-PCR检测海马内PSD95和SYP蛋白和基因的表达。结果与AD模型组相比,水迷宫检测显示中高剂量DADS组小鼠学习记忆能力增强,银染和电镜检测显示海马CA1区树突棘和突触增多,蛋白印迹和RT-PCR检测显示海马PSD95和SYP表达增多。结论随着DADS剂量的增加,AD小鼠的学习记忆能力得到改善,海马内树突棘和突触增多。

Objective To explore the effect of diallyl disulfide(DADS) on hippocampal synapses and learning and memory abilities in a mouse model of A1zheimer’s disease(AD). Methods Mouse models of AD established by agglutinated Aβ1- 42 injection in the lateral cerebral ventricle were randomized into 4 groups and treated with DADS at the daily doses of 0, 10, 50 and 100 mg/kg by gavage for 30 consecutive days. The learning and memory abilities of the mice were assessed with Morris water maze test; the structures of the dendritic spines and synapses in CA1 region of the hippocampus were observed under transmission electron microscope with silver staining; PSD95 and SYP protein and m RNA expressions in the hippocampus were detected with Western blotting and RT-PCR. Results Compared with the AD model mice, the mice treated with 50 and100 mg/kg DADS showed enhanced learning and memory abilities in Morris water maze test. The dendritic spines and synapses in CA1 region of the hippocampus increased obviously and hippocampal expressions of PSD95 and SYP were enhanced in mice treated with 50 and 100 mg/kg DADS. Conclusion DADS at the daily doses of 50 and 100 mg/kg can improve the learning and memory abilities and increase the number of dendritic spines and synapses in the hippocampus in mouse models of AD.