miR-501-5p在宣威地区肺腺癌组织中的表达及临床意义

Expression profile of miR-501-5p in lung adenocarcinoma patients from Xuanwei area

目的探讨24例宣威肺腺癌病人癌组织中miR-501-5p的表达与临床病理因素之间的关系及miR-501-5p在宣威地区肺腺癌中的作用。方法收集24例宣威地区肺腺癌患者的癌组织及相应的正常肺标本,提取组织中的微小RNA(microRNA),采用QPCR技术,microRNAs芯片扫描,检测miR-501-5p在肺癌组织和相应正常肺组织的表达量。使用χ2检验对miR-501-5P在宣威地区肺腺癌标本的表达量与临床特征因素(年龄,性别,肺癌分期及术前CEA值)进行统计学分析,运用多重回归分析miR-501-5p表达与临床特征(年龄,肺癌分期及术前CEA值)的关系。结果通过对24对宣威地区肺腺癌配对样本进行microRNA芯片检测和QPCR验证均提示:miR-501-5p在宣威地区肺腺癌中呈上调(P<0.01)。mciroRNA芯片结果(癌vs肺组织)表达差异倍数:3倍(P<0.05,FDR:0.07)。qPCR验证结果(癌vs正常肺组织)表达倍数(x±s):5.702±4.626。χ~2检验芯片miR-501-5p表达量与临床特征结果显示与年龄(f=7.168,P=0.014),肺癌TNM分期(f=36.627,P<0.01)及术前CEA值f=30.045,P<0.01)关系密切;但与性别(f=3.612,P=0.071)无关。在多重回归分析结果提示:miR-501-5p表达量与患者年龄,肺癌TNM分期、血清CEA(癌胚抗原)均呈正相关(P<0.05)。结论 miR-501-5p在宣威地区肺腺癌标本中呈高表达,其与年龄,临床肺癌TNM分期、血清CEA呈显著相关,可能参与宣威地区肺腺癌的发生发展。

Objective To investigate the relationship between miR-501-5p expression and the clinicopathological factors in patients with lung adenocarcinoma in Xuanwei area. Methods Surgical specimens of lung adenocarcinoma and paired adjacent tissues from 24 patients with lung adenocarcinoma from Xuanwei area were examined for miR-501-5p expression using microRNA microarray technique and qPCR. Chi-square test was used to analyze the association of miR-501-5P expression with the clinicopathological characteristics of the patients. Multiple regression analysis was performed to analyze the association of miR-501-5p expression with the patients' gender,age,tumor stage,and preoperative CEA level. Results Micro RNA microarray analysis and qPCR validation results revealed significantly upregulated expressions of miR-501-5p in patients with lung adenocarcinoma from Xuanwei area(P<0.01). The microarray data showed an up-regulation of miR-501-5p by 3.17 folds in lung adenocarcinoma tissue compared with the adjacent tissue(P=0.22376,FDR=0.071395). Chi-square test indicated that miR-501-5p expression level was associated with the patients' age(f=7.168,P=0.014),TNM stage(f=36.627,P<0.01),and preoperative serum CEA level(f=30.045,P<0.01),but not with the patients' gender(f=3.612,P=0.071). Multiple regression analysis revealed that miR-501-5p expression was positively correlated with the patients' age,TNM stage of the tumor,and serum CEA(P<0.05). Conclusion miR-501-5p expression is up-regulated in lung adenocarcinoma with significant associations with the patients' age,TNM stages and serum CEA level in patients from Xuanwei area,suggesting its potential role in the tumorigenesis and progression of lung adenocarcinoma in Xuanwei area.