抑制素基因疫苗的构建

Construction of inhibin gene vaccines

使用猪抑制素α亚基上的第 1- 32位氨基酸所对应的DNA片段作为目的基因 ,并与乙肝表面抗原基因串联 ,插入原核表达质粒 (pUCl8)中 ,构建重组原核表达质粒。经过在大肠杆菌中扩增后提取目的基因片段 ,插入真核表达质粒 (pcDNA3.0 )中 ,构建重组真核表达质粒。经过扩增和提取 ,并经过测定获得了抑制素基因疫苗。

Gene encoding inhibin α(1-32) was recombined with the gene hbs. The inhibin was inserted into pUC18 to construct recombined prokaryotic expression plastid. Target gene parts were extracted after expanded in E. coli and inserted into pc DNA 3.0 to construct recombined eukaryotic expression plastid. Inhibin gene vaccines were obtained through expansion, extraction and testing.