HMC毒素对雄花不育及正常细胞质玉米根冠活细胞的影响

Effect of HMC Toxin on the Living Root Cap Cells of Maize with Cms and N Cytoplasm

本文分析了三套(T、C、S、N)与20对(C、N)玉米同核异质系,在25℃条件下,经 C 毒素(HMC)处理3小时后,观察根冠细胞的死亡率。结果表明,C 细胞质的细胞死亡率均高于其它同核异质系。因此,C 毒素对 C 细胞质的毒害作用是特异性的,同时也为快速鉴定玉米雄花不育细胞质提供了一种新方法。

Three sets of inbred lines of maize with T、C、S、N cytoplasm respectively andtwenty pairs of inbred lines with N and C cytoplasm were studied for toxin C sensitiv-ity by the root cap cell viability test.The root cap cells were treated for 3 hr.withC-Toxin solution at 25℃.The results showed that the percentages of dead cells in va-riants with C cytoplasm was significantly higher than in those with N、S or Tcytoplasm but the same nuclei,indicating that the C toxin is specific to C cytoplasm,it also furnishes a rapid and sensitive method for the identification of different CMScytoplasm of maize.