应用丝瓜(Luffa Cylindrica Roem)伤流液提高水稻花药培养效果

Studies on the Effect of Bleeding Sap of Towel Gourd(Luffa Cylindrica Roem)on Rice Anther Culture

用15—20%(V/V)丝瓜伤流液加入培养基可以显著提高水稻花药培养力和花粉植株自然加倍的频率。诱导培养基中附加15—20%(V/V)丝瓜伤流液,形成愈伤组织百分率显著高于对照;粳稻的愈伤组织分化率高达95%,籼稻愈伤组织分化率可达70%,比对照提高1倍左右。花粉植株自然加倍的频率显著(p>0.01)高于对照。附加10%、15%、20%、25%(V/V)丝瓜伤流液诱导成的愈伤组织经继代培养后的重量除了25%(V/V)浓度外,其余都比对照增重5.7—18.09毫克/10块。绿苗的鲜重和干重分别增重20—89.0毫克/10株,13.0—30.0毫克/10株,在10—20(V/V)浓度的丝瓜伤流液范围内,对植物细胞分裂和生长有促进作用。其中以15—20%(V/V)浓度的丝瓜伤流液效果最好,经紫外光谱初步测定表明,丝瓜伤流液含有类似细胞激动素以及培养基中有机生长物质。

The anther culture ability and natural diploidization of regenerated plants of rice wereSignificantly increased by the addition of 15-20%(V/V)bleeding sap of towel gourd intothe induction medium,Thus,the callus induction rate of the Supplemented media wasSignificantly higher than that of the check;the plantlet differentiation rate was nearly doub-led,reaching 95% in Japonica rice and 70% in indica rice;and the natural diplodizationrate was highly Significantly higher(p>0.01).Further studies also revealed that Subculture on media Supplemental with 10%,15%and 20% (V/V) bleeding sap could lead to heavier weight in callus (5.7-18.0mg/10pieces of callus),heavier fresh weight(20-89mg/10 plantiets)and dry weight(13.9-30mg/10 plantlets)in green plantlets,whereasthe additionof 25% bleeding sapcould not bring about such improvement.These results indicated,therefore,thatwhich the range of 10-20%(V/V)Concentration,the addition of bleeding Sap oftowelgourd Could promote the cell division and growth of the rice plant,with 15%the optimum Concentration.