E-selectin和ICAM-1反义寡核苷酸对内皮细胞黏附肝癌细胞的影响

The Effects of E-Selectin and ICAM-1 Antisense Oligodeoxy Nucleotide on the Adhesion of Endothelial Cell to Hepatocarcinoma Cell

目的 :探讨反义寡核苷酸 (ASODN)对内皮细胞表达E selectin和ICAM 1的抑制及黏附肝癌细胞的影响。方法 :应用ASODN转染内皮细胞 ,通过流式细胞技术测定TNF α诱导后内皮细胞膜E selectin和ICAM 1的表达 ,RT PCR半定量测定E selectin和ICAM 1mRNA的表达 ,细胞黏附实验测定肝癌细胞与内皮细胞的黏附率。结果 :裸ASODN和脂质体 ASODN均降低了内皮细胞E selectin和ICAM 1分子及其mRNA的表达 ,后者尽管浓度低 ,作用更为明显 ;E selectin的ASODN显著降低了内皮细胞黏附肝癌细胞的黏附率。结论 :ASODN能竞争性地抑制内皮细胞黏附分子的表达 。

Objective: To explore the inhibition of E selectin and ICAM 1 expression in endothelial cells and the effects on adhesion of endothelial cells to hepatocarcinoma cells by antisense oligodeoxynucleotide(ASODN).Methods:With treatment of ASODN, the changes of E selectin and ICAM 1 protein expression in endothelial cells induced by TNF α were examined by flow cytometry, E selectin and ICAM 1 mRNA expression by RT PCR, and the adhesion rate of endothelial cells to hepatoma cells by cell adhesion experiment. Results: Both nude ASODN and liposome ASODN apparently brought down the expression of E selectin and ICAM 1 protein, as well as mRNA, and the latter worked better for all that lower dosage. E selectin ASODN significantly decreased the adhesion rate of endothelial cells to hepatocarcinoma cells.Conclusion: These data demonstratel that ASODNs were capable of selectively inhibiting the expression of E selectin and ICAM 1 in endothelial cells and thereby, to a certain extent, reducing the adhesion between endothelial cells and hepatocarcinoma cells.