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转染BMPⅡ型突变受体的Tca8113细胞的建立及意义 被引量：2

Gene tranfectkm of a truncated type n receptor of BMP - 2 into the Tca8113 cell
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摘要 目的 建立稳定表达BMPⅡ型突变受体的Tca8113细胞,以便进一步从信号转导水平探讨、认识BMPs对口腔上皮恶变的作用机理;方法 用FuGENE6 Transfection Reagent Kit真核转染试剂盒将携带BMPsⅡ型突变受体cDNA的真核表达载体转染Tca8113细胞;结果 得到抗G-418的阳性细胞克隆转染细胞,neo基因原位杂交阳性;结论 建立了稳定表达骨形成蛋白(BMPs)Ⅱ型突变受体的Tca8113细胞株。 Objective Toestablish a Tca8113 cell line which express a tuncated BMP - 2 a! receptor. Methods The cDNA of BMP - 2 type a!receptor was transfected into Tca8113 cells by using FuGENE6 Transfection eagent kit . Results Cell clones with G- 418 resistance and positive signals of mRNA of neo gene were obtained. Conclusion The cDNA for BMP - 2 type a! receptor is successfully transfected into Tca8113 cell.
作者 傅升 金岩 刘源 何黎升 赵宇
机构地区 第四军医大学口腔医学院
出处 《临床口腔医学杂志》 2002年第5期325-327,共3页 Journal of Clinical Stomatology
关键词 BMPⅡ型突变受体 TCA8113细胞 骨形成蛋白 转染 舌鳞癌 发病机制 Tca8113 cell Bone morphogenetic protein Receptor Transfection
分类号 R739.86 [医药卫生—肿瘤]
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参考文献11

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同被引文献22

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临床口腔医学杂志
2002年 第5期

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