p53、nm23基因和端粒酶表达与非小细胞肺癌临床病理关系的探讨

An appraisal on the relevance of expressions of p53 gene, nm23 gene and telomerase with the clinicopathology of non-small cell lung cancer

目的 探讨癌基因、端粒酶与非小细胞肺癌临床病理特征的关系。方法 应用免疫组化SP法和PCR TRAP法对 40例非小细胞肺癌行p5 3、nm2 3和端粒酶检测。结果 癌组织中阳性反应检出率分别为3 7.5 %、17.5 %和 92 .5 % ;临床及病理TNM分期早期肺癌p5 3基因表达明显高于中、晚期肺癌 (P <0 .0 5 ) ;nm2 3基因表达与肺癌的组织学类型、淋巴结转移和病理TNM分期均无关。 60岁以下肺癌患者的端粒酶阳性率明显高于 60和 60岁以上的肺癌患者 (P <0 .0 5 ) ;60岁以下肺癌患者淋巴结转移率及病理TNM分期中、晚期比率略高于 60和 60岁以上的肺癌患者 ,分别为 69.2 %、5 0 %和 76.9%、5 7.1% (P >0 .0 5 ) ;p5 3、nm2 3和端粒酶阳性表达无协同性 (P >0 .0 5 )。结论 p5 3阳性表达可作为非小细胞肺癌的早期诊断和高危人群的筛选指标 ;nm2 3与肺癌的发生、发展和淋巴结转移均无关 ;端粒酶阳性表达与肺癌患者的年龄密切相关 ,可能与肺癌的发生、发展和转移有关 ;p5 3、nm2

Objective To explore the relevance of oncogenes, telomerase with the clinicopathologic characteristics of non small cell lung cancer. Methods The expressions of p53, nm23 and telomerase were studied in 40 NSCLC cases by SP and PCR TRAP methods. Results The positive rates of p53, nm23 and telomerase were 37.5%, 17.5% and 92.5% respectively. The expressions of p53 in early stage of T and P TNM were significantly higher than that in the middle and advanced stage( P <0.05). No significant correlations could be found between nm23 expression and the histologic type, lymph nodal metastasis and pathologic staging( P >0.05). The expression of telomerase below 60 year old patients was significantly higher than that in the controlled group( P <0.05). The rates of lymph nodal metastasis and middle, advanced pathologic staging below 60 year old patients were higher than that in the controlled group, 69.2% vs 50% and 76.9% vs 57.1% respectively( P >0.05). There were no significant relationship between p53, nm23 genes and telomerase( P >0.05). Conclusions The expression of p53 can be an early diagnostic and screening method of NSCLC. There is no significant correlation between nm23 expression and histologic type, lymph nodal metastasis or pathologic staging. Telomerase activity is significant related with age and may be implicative in the carcinogenesis and metastasis. There is no significant relationship between p53, nm23 and telomerase.