幽门螺杆菌毒力因子与胃癌前变化的研究

Study of virulent factor of Helicobacter pylori with the gastric precancerous changes

目的 研究胃癌前状态和癌前病变伴幽门螺杆菌 (Hp)感染的患者 ,其Hp毒力因子细胞毒素相关蛋白A(CagA)、空泡细胞毒素VacA的表达。方法 常规胃镜检查和活检取得的胃黏膜标本做组织病理检查 ,根据所得结果 ,慢性萎缩性胃炎、胃息肉、残胃、胃溃疡、完全型 /不完全型、大肠 /小肠化生为癌前状态组 (82例 ) ,胃黏膜上皮异型增生为癌前病变组 (2 5例 ) ,单纯慢性浅表性胃炎伴Hp感染患者为对照组 (3 0例 )。用蛋白印迹法 (WesternBlot)测定Hp的CagA、VacA因子。结果 癌前状态组和癌前病变组中 ,CagA、VacA因子的检出率显著高于对照组 (P <0 .0 5 )。结论 幽门螺杆菌CagA。

Objective To investigate the correlation of virulent factors CagA,VacA of Helicobacter pylori and the gastric precancerous lesion. Methods All patients were performed routine gastroscopy and biopsy, 82 patients with gastric precancerous condition and Helicobacter pylori infection taken with as a group consisted of chronic atrophic gastritis(CAG), gastric polyp, gastric remnant, gastric ulcer(GU) and complete/ incomplete colonic/smallintestinal metaplasia(IM). 25 patients with gastric precancerous lesions(dysplasia) and Helicobacter pylori infection formed another group, and 30 patients with chronic superficial gastritis and Helicobacter pylori were assumed in the control group. The serum of Helicobacter pylori CagA, VacA proteins were analyzed by Western blot. Results In the groups of gastric precancerous condition and gastric precancerous lesion, positive CagA, VacA reaction was significantly greater than those of the control( P <0.05). Conclusion CagA, VacA factor bears a close relationship with the gastric precancerous condition and gastric precancerous lesions.