我国疫区HFRS患者汉滩病毒核蛋白特异性CTL克隆的建立

A study on CTL clone with specificity of Hantaan virus nucleocapsid protein from peripheral blood of patients with HFRS

目的 探讨汉滩病毒核衣壳蛋白 (HTNV NP)诱生的特异性细胞免疫的分子基础 ,阐明HFRS的发病机理。方法 分离HFRS患者的PBMC ,建立HTNV NP特异性的CTL克隆 ,同时将克隆有HTNVS基因不同片段的真核表达载体转染患者自身的B淋巴母细胞系 (BLCL) ,建立CTL靶细胞系 ,并进行细胞杀伤试验。结果 建立的特异性CTL克隆对表达完整NP、NP羧基和氨基端肽段的靶细胞均有比较明显的杀伤效应 ,平均杀伤率分别为 5 0 .2 %、39.0 %和 2 5 .4 %。结论 HTNV NP优势T细胞表位可能主要位于病毒核蛋白的羧基端。

Objective To identify the immunological mechanism and pathogenesis of HFRS. Methods We isolated PBMC from patients with HFRS and established CD8 + cytotoxic T lymphocyte (CTL) clone directly from peripheral blood mononuclear cells of a patient of acute hemorrhagic fever with renal syndrome (HFRS). CTL cytotoxic assays were performed as usual using EBV transformed lymphoblastoid cell lines (BLCL) as target cells. Results The CTL clone recognized and killed target cells with specificities of HTNV NP. Cytotoxicities percentage were 50.2%, 39.0% and 25.4% respectively. Conclusion HTNV T cell antigenic epitopes mainly localize on the C terminal of the virus nucleocapsid protein. [