检测病毒性肝炎患者血清中SEN病毒及其临床意义

SEN virus and its clinical significance in patients with viral hepatitis

目的 检测病毒性肝炎患者血清中SEN病毒D和H(SENV D、SENV H) ,并探讨其临床意义。方法 采用巢式聚合酶链反应法 (nPCR)检测甲、乙、丙、戊型肝炎和非甲～戊型肝炎患者血清中SENV D和SENV HDNA。结果 在 180例病毒性肝炎患者血清中 ,SENV D和SENV H检出率分别为 17.2 % (31 180 )和 5 .6 % (10 180 ) ,总检出率为 18.3% (33 180 )。甲、乙、丙、戊型肝炎患者的SENV D H检出率高于非甲～戊型肝炎患者。从甲、乙、丙、戊型肝炎和非甲～戊型肝炎患者中分离的SENV D H核苷酸序列 ,与SENV D H原型株比较 ,其同源性在 94 %以上。甲、乙、丙和戊型肝炎患者有无SENV D H合并感染 ,其血清生化学指标无明显差异。结论 SENV D H可能不是非甲～戊型肝炎的病原 ,甲、乙、丙和戊型肝炎患者合并感染SENV D H并不加重病情。

Objective To detect SEN virus D and H (SENV D, SENV H) in serum from patients with viral hepatitis and its clinical significance. Methods SENV D and SENV H were detected by a nest polymerase chain reaction(nPCR) in the sera of patients with hepatitis A, B, C, E and non A to E (HAV, HBV, HCV, HEV and non A E). Results Of 180 patients with viral hepatitis including 30 acute hepatitis A, 30 chronic hepatitis B, 30 chronic hepatitis C, 30 acute hepatitis E, and 60 non A to E hepatitis, 31(17.2%) were SENV D positive, and 10(5.6%)were SENV H positive. The total positive rate of SENV D/H was 18.3% (33/180). The SENV D/H positive rate of patients with HAV, HBV, HCV and HEV hepatitis was higher than that with non A E hepatitis. The nucleotide homology between SENV H/D prototype and isolates from patients with viral hepatitis was more than 94%. No difference was found in biochemical markers between hepatitis A, B, C, E patients with SENV D/H infection and the patients without SENV D/H infection. Conclusion SENV D/H is not a pathogenic agent of non A E hepatitis, and SENV D/H coinfected with HAV, HBV, HCV and HEV does not contribute to the severity of the diseases. [