水酶法提取玉米胚油工艺

Study on Aqueous Enzymatic Extraction of Corn Germ Oil

对采用水酶法从玉米胚中提油的工艺及其加工参数进行了实验研究 ,结果表明 :将玉米胚浸泡于 0 .5mol/L、pH 4的柠檬酸缓冲液中 ,经 10 0℃ ,4 0min热处理 ,添加 2 % (质量分数 )的纤维素酶 ,反应 7h ,清油提取率为 78.72 % ,总油提取率为 88.18% .

A new technique for aqueous enzymatic extraction of corn germ oil was developed. Influence of pH and ion strength of buffer on the extraction of protein and total sugar was studied. Yield of free oil and total oil depended greatly on pH, ion strength of buffer, temperature,time of HTT(hydrothermal treatment), time of conversion, and quantity of enzyme. 79.72% free oil and 88.18% total oil were obtained when corn germ was soaked in 0.5 mol/L,pH 4 citrate buffer, followed by HTT at 100 ℃ for 40 min,then hydrolyzed for 7 h by 2% cellulase.