饲料粗蛋白快速测定法研究

Study on the Method for Rapidly Determining Crude Protein in Feeds

研究了饲料粗蛋白质快速测定方法 ,利用高氯酸和硒粉相结合缩短凯氏定氮法消化时间 ,使饲料样品在1min左右消化成无色或黄色溶液。在继续加热完成消化时 ,利用淀粉防止残余高氯酸引起的高温氮损失。本方法测量结果与标准法相比较 ,相对误差大多在 3 %以内 ,消化样品所需时间约为 2 0min。

For rapidly determining crude protein in feeds perchloric acid and selenium powder were used in Kjeldahl N determination to reduce the digestion time of samples, which were digested into a colorless or yellow solution in about one minute. When the solution was heated for further digestion, starch was used to prevent the N loss caused by perchloric acid under high temperature. The relative deviatons were mostly within 3% in comparison with the results from the standard method, and the time needed for sample digestion was about 20 min.