聚合酶链反应及斑点杂交技术对喉尖锐湿疣复发原因的探讨

Detection of human papillomavirusrelated sequence from recurringcondyloma acuminata oflarynx by GP-PCR andhybridization

用通用引物介导的聚合酶链反应及其产物斑点杂交技术,对复发性喉尖锐湿疣组织中人乳头状瘤病毒(HPV)相关序列进行了检测和分型,结果例3次手术标本均为HPV6型,表明隐性感染和/或原发病灶未彻底清除,可能是喉尖锐湿疣复发的重要原因,因此建议手术时适当增大切除范围。

Operative specimens from recurring condyloma acuminata (CA) of larynx were analyzed for HPV DNA related sequence by general primer-mediated polymerase chain reaction (GP-PGR) and dot blot hybridization. Threet imes operation specimens from a case were found to harbor HPV6 DNA homologous sequence. It indicates that latent infection and/or the remainder of primary infection may be responsible for the relapse.