耳蜗及前庭组织培养法的建立

Tissue culture of organ of Corti and utricle

目的为建立耳蜗Corti器及椭圆囊体外培养实验模型。方法离休Corti器及椭圆囊组织分别在DMEM+胰岛素+20%胎牛血清+葡萄糖培养基中培养9天和Medium-199+10%胎牛血清培养基中培养20天,并与同期体内发育结果比较。结果培养组织生长良好,组织结构及细胞形态均得到较好保存,其细胞分化程度与体内同期类似,无退化现象。结论该方法不仅能使组织结构及细胞形态得到保存,而且保留了细胞的活性,为耳毒性药物的作用及机制、毛细胞再生研究提供了有实用价值的实验模型。

METHODS Explants of the organ of Corti have been maintained 9 days in vitro (DIV). They have grown well in DEME medium containing 20% fetal serum (FBS) and supplemented with insulin (Gibco) and glucose. Tissues of utricle have been maintained 20 DIV. They have grown well in Medium-199 containing 10% FBS. Morphology and structure in vitro have been constrasted with those in vivo.RESULTS These models can retain tissue and cell morphological and struc-trual integrity. The histological differentiation of the organ proceeding in vitro is similar to that in vivo.CONCLUSIONS This methods of tissue culture may retain their structralorganization and most of the hair cells are alive in their differentiation. It provides a good tool for studying the regeneration of hair cells and the effect of ototoxic drugs.