喉鳞癌的一氧化氮合酶免疫组化特性

Immunohistochemical characterization of nitric oxide synthase in laryngeal squamous cell carcinoma

目的检测喉鳞癌组织的一氧化氮合酶(NOS)表达情况,并分析其表达与喉鳞癌病理特性关系。方法应用免疫组织化学技术检测20例喉鳞癌、15例声带息肉、5例咽粘膜组织的NOS表达。结果声带息肉及咽粘膜组织iNOS eNOS表达阴性,喉鳞癌iNOS的表达强度与临床分期无关;低分化癌与高分化癌iNOS的表达强度差异有显著性(P<0.05),低分化癌与高分化癌eNOS的表达阳性率差异有显著性(P<0.05)。结论 iNOS的表达可能为肿瘤细胞间的异常介质信号,与喉鳞癌的发生、分化有关。

PURPOSE To study the expression of nitric oxide synthase(NOS)in laryngeal carcinomas(LC),and analyze the relationship between their expression and the pathological character.METHODS Using immunohistochemical technique to determine the expression of NOS in 20 cases of LC,15 cases of polyp of vocal cord and 5 cases of pharyngeal mu-cosa.RESULTS The iNOS eNOS expression of polyp of vocal cord and pharyngeal mucosa was negative.The relationship between iNOS expression intensity and clinical staging had no statistic significance,the expression intensity of the lower differentiation squamous carcinomas was higher than that of the higher differentiation(P&lt;0.05),and the postitive rate of expression of eNOS in the lower differentiation squamous carcinomas was higher than that of the higher differentiation(P&lt;0.05).CONCLUSIONS Expression of NOS might relate to pathogensis and differentiation of laryngeal cancer.