颈侧进路咽鼓管阻断法建立豚鼠渗出性中耳炎模型

Establishment of guinea pig model of secretory otitis media with eustachian tube obstruction

目的为渗出性中耳炎的实验研究提供可靠的动物模型。方法本实验以豚鼠为实验对象,国内首次运用颈侧进路阻断咽鼓管法建立渗出性中耳炎动物模型。结果发现:1.大体观察术后7天即产生听泡积液,并于2周达到高峰。2.光镜观察术侧听泡粘膜及粘膜下层进行性增厚,同时表现以浆细胞浸润为主的慢性炎症过程。结论颈例进路咽鼓管阻断法是一种能稳定造成中耳积液的渗出性中耳炎的造模方法,不仅安全、快捷,而且适用于小动物如豚鼠,因而易于推广。

PURPOSE To provide a reliable animal model for experimental researches on SOM.METHODS Albican guinea pigs were adopted as experimental subjects. The surgical techniques of external cervical approach to obstruct eustachian tube were invented and employed first time domestically to establish SOM animal models.RESULTS Our results demonstrated: 1. Observation by naked eye: middle ear effusions appeared 7 days later immediate to surgery, and peaked at 2 weeks later. 2. Microscopic observation: the depths of middle ear mu-cosa and submucosal layer were found to be progressively increased and chronic inflammatory courses characterized by monocytes infiltration were found to be obvious in operated otic capsule cavity. CONCLUSION Our results showed that the external cervical approach to obstruct eustachian tube was an ideal method of making animal model of SOM stably and easy to popularize, not only because of its safty and swiftness of the manipulation, but also of easily available small animal resources such as guinea pigs.