谷氨酸发酵噬菌体研究初报

Primary Study on Phages of Glutamic Acid Fermentation

噬菌体污染,常给谷氨酸发酵造成严重危害,所以查明系外源噬菌体的污染或溶原菌诱导出的噬菌体污染,就成了人们关注的首要问题。我们从谷氨酸生产菌北京棒状杆菌突变株——FM820-7紫外线、丝裂霉素C诱导分离到二株噬菌体526,528,从FM820-7菌株生产环境样品中分离到一株噬菌体530,对分离的噬菌体形态及生物学特性进行了初步研究。并就溶原菌株诱导方式、噬菌体的检测实验条件进行了探讨,为迅速澄清污染源,提供了直接有效的手段。

Phage contamination often bring serious damage in producing glutamic acid. The first concern of us is to find out whether the cause of contamination is from natural environmental phages or from the phages produced by the induction of lyso-gen. Two phages have been obtained by UV and mitomycin C induction from FM820 -7, the mutant of Corynebacterium pekinese (glutamate-producing bacteria) and designated as 526 and 528 respectively. Another phage was isolated from the fermentation process of FM 820-7 and designated as 530. Morphology and biological properties of these phages as well as the conditions of phage replication and phage release were studied. Adjusting the concentrations of Ca++ and Fe++of the liquid medium could be used for direct and efficient inhibition of phage contamination.