爆震对听阈、血管纹Na^+-K^+-ATP酶、琥珀酸脱氢酶的早期影响

Early effects of explosion on hearing threshold and activities of Na^+-k^+-ATPase and succinic dehydrogenase in the stria vascularis of guinea pig

本实验观察了爆震后90分钟豚鼠听阈、血管纹Na^+-K^+-ATP酶、琥珀酸酸脱氢酶(SDH)、血管纹截面积及耳蜗形态的变化,讨论了其间的关系及爆震性耳聋的致伤机理。

Slow cortex response to several frequencies was recorded in guinea pigs bofore and after intense impulse noise exposure. Guinea pigs were decapitated 90 minutes after the exposure. Besides scanning electron microscopic examination of cochleas, activities of Na^+-K+-ATPase and SDH in the stria vascularis(SV) corresponding to 4 kHz were studied with histochemical methods under light microscope. Densities of the histochemical reaction products and cross sections of the SV were measured with Image Analysis System. Activities of Na^+-K^+-ATPase and SDH in the SV decreased significantly in PTS group(P<0.01, P<0.05). There was no significant change in TTS group. It is suggested that the decrease of Na^+-K^+-ATPase and SDH in the SV may contribute to remarkable hearing threshold shift besides mechanical destruction by noise. Metabolic disturbance may aggravate hair cell damage. Direct damage of the SV caused by intense noise may be one of the causes of PTS.