摘要
以抗 HCVNS3的单克隆抗体作为固相筛选分子 ,对人工合成的噬菌体随机 12肽库进行 5轮“吸附 洗脱 扩增”的筛选过程 ,随机挑取 4 2个克隆 ,经噬菌体酶联免疫吸附法 (ELISA)鉴定并进行交叉反应实验以及竞争抑制性结合实验 ,最后对所选克隆进行DNA序列分析 ,以确定HCVNS3抗原的模拟表位。经噬菌体富集后 ,从随机筛选的 4 2个克隆中得到 11个阳性克隆 ,确定氨基酸序列XXIXXXXMSNXX为HCVNS3的模拟表位。我们用噬菌体12肽库成功筛选得到HCVNS3的模拟表位 。
Using HCV NS3 monoclonal antibody as selective molecule, a 12 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. Eleven positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as mimotope of HCV NS3. HCV NS3 mimotope was obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.
出处
《中国病毒学》
CSCD
2002年第3期202-205,共4页
Virologica Sinica
基金
国家自然科学基金资助项目 (3 990 0 13 0 )
