污灌对小麦幼苗生长及活性氧代谢的影响

Effect of sewage irrigation on wheat growth and its activate oxygen metabolism.

通过水培的方式研究了污水灌溉对小麦幼苗生长及活性氧代谢系统的影响 .结果表明 ,经污水浇灌的小麦幼苗与对照组相比 ,植株矮小 ,根短 ,根数目少 ,茎、叶、根的干重、鲜重和可溶性蛋白含量均明显减少 ,并出现叶尖枯黄 ,叶片色素含量下降 ,叶绿素a/b与电导率明显上升现象 ,几种色素对污灌的敏感性为叶绿素b >叶绿素a >类葫萝卜素 .同时MDA水平上升 ,而SOD、POD和CAT活性显著下降 .其中SOD对环境胁迫最敏感 .三者活性分别下降 19.1%～ 33.1%、37.8%～ 4 7.8%和 30 .2 %～ 35 .9% .

The effects of sewage irrigation on the seedling growth of wheat and its activate oxygen metabolism were studied by water cultivation experiments .The results showed that compared with control,the wheat under sewage irrigation was short and small, and its roots number,fresh /dry weight of stem or root,and content of soluble protein decreased drastically.The content of chlorophyll decreased, while the Cyta/Cytb and membrane lipid peroxidation were increased. The sensitivity of pigment content of wheat leaf undr sewage irrigation was in order of Chlorophyll b > Chlorophyll a >Carotenoid.Furthermore, the POD, SOD and CAT activities of the wheat went down in varying degrees,and the SOD was the most susceptible enzyme to waste water stress. In this paper, the activities of POD, SOD and CAT decreased in 19.1%～33.1%,37.8%～47.8% and 30.2%～ 35.9%,respectively.