大肠埃希菌表达苏云金杆菌cryIVD基因杀蚊幼活性观察

Observation on Larvicidal Activity Against Mosquitoes with B.t.i cryIVD Protein Expressed in E.coli DH5 α

目的 :观察表达B .t.icryIVD基因的大肠埃希菌对蚊幼虫的毒效。 方法 :细菌的诱导表达及蚊虫毒效测试。结果 :加入工程菌 48h对淡色库蚊和白纹伊蚊均具有明显的杀灭作用 ,LC50 分别为 2 .3 8× 10 6 、1.6× 10 7个 ml细胞 ,其中对淡色库蚊的杀灭作用要好于对白纹伊蚊幼虫的杀灭效果。结论 :单独表达B .t .

Objective:To observe the larvicidal activity against mosquitoes with B.t.i cryIVD protein expressed in E.coli . Methods:Induced by IPTG and detection of larvicidal activity. Results:The engineered E.coli had larvicidal activity against Culex pipiens pallens and Ae.albopictus. Conclusion:The B.t.i cryIVD protein expressed in E.coli DH5 α alone showed larvicidal activity against mosquitoes.