O157∶H7宿主动物及传染源3年监测研究

A 3-year Surveillance Study of host Animals and Infectious Source with E.coli O157∶H7

目的 :探明家禽家畜的O15 7∶H7感染与饲养情况。方法 :用细菌学与PCR等技术检测O15 7∶H7及其毒力基因。结果 :从牛、羊、猪、鸡的粪便中检出O15 7∶H7及其毒力基因VT2 、eaeA和hly ,带菌率的高低依次为牛 >羊 >鸡 >猪 ,腹泻病流行前期禽畜的带菌率高于流行期 ;该地农村禽畜饲养密度高 ,户饲养数 10 .2只 (头 ) ,人均 2 .4只 (头 ) ,饲养方式以散养为主 ,散养率 87.76%。结论 :牛、羊、猪、鸡是该地O15 7∶H 7的主要宿主动物和传染源 ,加强对其实行良好的饲养方式与粪便无害化管理及注重个人与环境卫生是防治O15

Objective:To investigate the rate of infection with E.coli O157∶H7 and feed conditions in poultry and mammals in the Donghai county, Jiangsu province, China. Methods:To determine presence of E.coli O157∶H7 and its toxic genes VT 2, eaeA and hly with bacteriological method and PCR. Results: E.coli O157∶H7 and its toxic genes VT 2, eaeA and hly were detected in cow, sheep, pig, and chicken excretions. The prevalence of infection with E.coli O157∶H7 was observed in the decreasing order in cows, sheep, chicken, and pigs. In addition, the prevalence was higher in the pre-epidemic phase than epidemic phase. Increased rate of infection was also associated with higher density of feeding poultry and mammals in this rural area. Each family fed 10.2 animals, averaging 2.4 animals fed by each pergon. 87.76 % of the animals were fed natural environmental diet. Conclusion:Main host animals infected with E.coli O157∶H7 are cows, sheep, pigs, and chicken. Prevention of bacterial transmission from host animals to humans requires decontamination of animal feed, proper removal of animal excretions, and increased personal hygiene.