染色体-质粒平衡致死系统的构建及应用

The construction and application of chromsome-plsmid balanced-lethal system

以营养选择标志代替抗生素抗性标志为主要特征的染色体-质粒平衡致死系统的建立为基因工程产品的生产和基因治疗提供了更为安全,有效的操作平台。通过质粒载体基本元件与宿主菌之间的优化,构建出用于不同目的的外源基因的平衡致死系统。它对构建单价或多价减毒活菌苗起到了至关重要的作用;随着基因疫苗研究的深入,使它具有更为广阔的应用前景。本文着重讨论目前该系统的构建方法和应用。

Characterized by the nutritional selector marker instead of the drug-resistant one,the construction of chromsome-plasmid balanced-lethal system can provide a safer and more efficent interface to manipulate the gene product and gene therapy.The optimization of plasmid basic units and the host bacteria enables the versatility of heterologous gene balanced-lethal system,which is of importance in the application of single or multiple value aviru-lent live strains.With the understanding of gene vaccine deepened continuously,the balanced-lethal system will hold greater potential in gene engineering.In this review,we focused on the method and the application of it.