摘要
以浙江天台山大血藤为材料 ,对其DNA提取和RAPD分子标记方法进行了研究。结果表明 :所采用的经改进的SDS提取法可获得高质量的大血藤DNA ,分子量大于 2 3kb ,可满足RPAD扩增 ;用 1 5个不同的随机引物对所提取的 1 2个个体的大血藤DNA进行了RAPD分子标记分析 ,其中 1 4个引物扩增产物具有多态性。建立了大血藤DNA提取和RAPD标记的分析程序 。
DNA extraction and RAPD amplification of Sargentodoxa cuneata was studied. The results showed that the good quality DNA of Sargentodoxa cuneata was gained using improved SDS method and the length of DNA fragment was more than 23kb. The isolatd DNA could be used for RAPD analyses with more purification. RAPD markers were examined in 12 individuals Sargentodoxa cuneata , and 14 of 15 primers reveal polymorphisms. The RAPD analysis procedure in this experiment lays a good foundation for application of RAPD in Sargentodoxa cuneata genetic research.
出处
《植物研究》
CAS
CSCD
北大核心
2002年第4期483-486,共4页
Bulletin of Botanical Research
基金
国家自然科学基金资助项目 ( 3 9870 160 )
