大肠癌细胞DNA含量与病理形态学及预后的关系

DNA content and its relationship with pathology and prognosis in colorectal carcinoma

本文应用流式细胞仪检测了181例常规福尔马林固定石蜡包埋的大肠癌组织标本的肿瘤细胞DNA含量。结果表明:大肠癌细胞DNA含量与组织类型及分化程度有关。低分化腺癌、粘液腺癌和管状腺癌异倍体比例明显高于乳头状腺癌;异倍体肿瘤随Dukes分期增高而增加;Cox回归模型分析表明:肿瘤倍性、Dukes分期和肿瘸大小明显影响预后。结果提示大肠癌细胞DNA含量反映了肿瘤的恶性程度,明显影响了患者的预后,可以作为判断患者预后的指标之一。

The DNA content of 181 colorectal cancers was measured by flow cytometry on paraffin-embedded specimens. Eighty five (46.96%) were diploid, 96(53.04%) were aneuploid. The proportions of aneuploidy were significantly lower in the patients with Dukes'stage A(25.00%) and B (47.14%) than in the patients with Dukes'stage C(64.71%) and D(68.42%). Associations were also found between ploidy and other discreted pathologic variables: histologic type and grade. The cumulated five-years survival was 66.67% and 33.80% for the patients with diploid and aneuplold tumors (P<0.01). Analysis of Cox regression revealed that DNA pattern, Dukes'stage and tumor size significantly influenced patients' prognosis. It is suggested that DNA pattern may be an important prognostic factor in colorectal cancer.