应用RT-PCR技术检测葡萄扇叶病毒的研究

Detection of Grapevine Fan Leaf Virus by Reverse Transcription-Polymerase Chain Reaction

以扇叶病株为材料 ,根据病毒外壳蛋白核苷酸序列 ,设计特异引物P1(10 6 4 10 83) ,P2 (76 2 781) ,建立了以RNA为模板的GFLV的RT

Research on an advanced method for GFLV detection.RT PCR primers were selected from the GFLV coat protein, P\-1 (1064 1083), P\-2(762 781). RT PCR can be carried out using RNA as template.In virtue of short period,high accurate, think of the potential value on further application .