内毒素肝损伤过程中枯否细胞NF-κB和AP-1活性变化及其生物学意义

Kinetics and Significance of the Activation of NF-κB and AP-1 in KCs During LPS-induced Liver Injury

为探讨内毒素肝损伤过程中 ,枯否细胞 (Kupffercells,KCs)内主要转录因子Nuclearfactor kappaB(NF κB)、Activatorprotein 1 (AP 1 )活性的动态变化规律 ,采用健康昆明种小鼠 ,随机分组如下 :Lipopolysaccharide(LPS)尾静脉注射 3h的量效关系 :正常对照组和低 ( 1mg kg)、中 ( 5mg kg)、高 ( 1 0mg kg) 3个内毒素剂量组 ;注射 5mg kgLPS的时效关系 :正常对照、0 .5、1、3、5、8h组 .Electrophoreticmobilityshiftassay(EMSA)法检测KCs中NF κB、AP 1活性 .结果发现 ,内毒素肝损伤过程中 ,KCs中NF κB、AP 1在不同时效和量效均不同程度活化 .提示 ,二者的活化与炎症反应的调控机制紧密相关 。

To explore the kinetics of the activation of Nuclear factor-kappa B(NF-κB) and Activator protein-1(AP-1),Kunming mice were divided randomly as follows:does-effect of Lipopolysaccharide(LPS) infusion 3 h:normal control group,low(1?mg/kg),middling(5?mg/kg),high(10?mg/kg)LPS induced groups;time-effect of 5?mg/kg LPS infusion:normal control group,0.5,1,3,5,8?h groups.NF-κB and AP-1 activities of KCs were determined with Electrophoretic mobility shift assay(EMSA).We found that NF-κB and AP-1 in KCs were activated remarkably during LPS-induced liver injury.It showed that the activation of NF-κB and AP-1 is closely connected with the regulation of inflammation and the inhibition of their activation may be used in inflammatory therapy.