HSF2 mRNA在热应激和大剂量11酸睾酮诱导恒河猴生精细胞凋亡中的表达变化

CHANGES OF HSF mRNA EXPRESSION IN RHESUS MONKEY TESTES DURING GERM CELL APOPTOSIS INDUCED BY HEAT STRESS OR HIGH DOSAGE OF TESTOSTERONE UNDECANOATE

为了探讨HSF2mRNA在热应激和超生理剂量睾酮诱导恒河猴生精细胞凋亡中的表达变化 ,我们建立了手术诱导单侧隐睾和注射大剂量 11酸睾酮 (TU)恒河猴动物模型 ,应用 3′末端标记分析 (TUNEL)和原位杂交方法 ,检测睾丸细胞的凋亡信号和HSF2的表达变化。TUNEL结果显示热应激和超生理剂量睾酮能够诱导生精细胞出现凋亡信号 ,它分别于处理后第 5天和第 30天达到最强 ,表明热应激和睾酮干扰精子发生可能是通过生精细胞凋亡的方式来实现的。HSF2mRNA水平在生精细胞凋亡早期 (凋亡信号达到最强以前 )略有降低 ,而在凋亡高峰期之后其表达急剧下降。Hsf2基因与我们以前研究的Hsp70 2基因的表达具有时间上的相关性 ,表明HSF2蛋白可能调控Hsp70 2基因的表达 ,而且HSF2可能通过多种方式影响精子的发生以及抑制生精细胞的凋亡。

In order to investigate the changes of HSF2 mRNA expression and its role in germ cell apoptosis induced by heat stress or a high of testosterone undecanoate,we established the operation induced unilateral cryptorchid and TU injected monkey models,and examined in situ analysis of testicular cell DNA fragmentation and the expre ssion level of HSF2 mRNA in germ cells after treatment.The TUNEL results showed that the apoptotic signals after treatment increased and the strongest apoptotic signals appeared in the germ cells of the cryptorchid testes on day 5 or the TU treated monkey testes on day 30,suggesting that the failure of spermatogenesis in the testes of monkeys after treatment may be due to the germ cell apoptosis induced by heat stress or high TU.The HSF2 mRNA decreased slightly at the early phase of the cell apoptosis just prior to the strongest signals of apoptosis,followed by dramatic decrease.This change was closely time correlated to that of Hsp70 2 mRNA(unpublished observation).It, therefore, suggests that HSF2 may regulate the expression of Hsp70 2.Moreover,HSF2 may control the spermatogenesis and the germ cell apoptosis by other signal pathways.