佳禾早占成熟种胚高效率组织培养方法的研究(简报)

STUDY ON HIGH EFFICIENCY TISSUE CULTURE OF MATURE SEEDS OF RICE(JiaHe-ZaoZhan)

水稻作为最主要的粮食作物之一,其产量和质量都倍受人们关注,通过将一些优良基因导入水稻,或是通过物理、化学等手段诱变,培养优质、高产、多抗新品种是现代育种科学的发展方向。但无论是基因片段的转入或是物理、化学的诱变处理,其对象多是具有分化再生能力的愈伤组织。自1965年以来,研究者们先后以水稻各部位为材料,诱导出愈伤组织和再生出植株,取得了较好的结果。但由于这些材料的来源受季节的限制,取材不便。

In order to improve the embryogenic callus induction rale and the regeneration rate of JiaHe-ZaoZhan rice, the influence of different factors were investigated, media with different hormones were used. Induction medium was supplemented with 1. 5mg/L 2,4-D + 3 mg/L NAA +0.1 mg/L KT + 1 mg/L phytic acid+ 20mg/L PAA. Embryogenic call were treated under the condition of 25 ℃ before transferring to regeneration medium, the regeneration medium contained 0.5 mg/L 6-BA + 3 mg/L NAA +0.5 mg/L KT + 1 mg/L phytic acid. The experiment results indicated that the hormone treatments had certain effects on the callus induction. Under the optimal medium, culture condition and the hormone treatments, the embryogenic callus induction could reach over 95 % , and dry treatment of embryogenic callus had been found to increase the frequency of plant regeneration, significantly the plant regeneration rate could reach over 80%. Transplanted into pots , the young plants grew well. Then a experimental system with stability and high regenerating efficiency has been established for the mature seeds of rice (JiaHe-ZaoZhan).