期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

尾加压素对新生大鼠心肌细胞一氧化氮合成的影响 被引量：7

Effect of urotensin n on the nitric oxide production in neonatal rat cardiomyocytes
下载PDF
导出
摘要 应用半定量逆转录-多聚酶链反应法,观察尾加压素（urotensinⅡ,UⅡ）对培养的新生SD大鼠心肌细胞内皮型一氧化氮合酶（endothelial nitric oxide synthase,eNOS）mRNA表达的影响,并测定UⅡ对心肌细胞内一氧化氮合酶（nitric oxide synthase,NOS）活性和一氧化氮（nitric oxide,NO）释放的影响。结果显示:UⅡ抑制培养的新生大鼠心肌细胞eNOS mRNA表达、抑制NOS的活性及NO释放;0.1μmol/L浓度的UⅡ呈时间依赖性抑制心肌细胞NOS的活性及NO生成。上述实验结果提示UⅡ的心血管作用可能与NO合成系统有关。 The aim of this study was to investigate the effect of urotensin Ⅱ(U Ⅱ) on the nitric oxide (NO) production in cultured neonatal rat cardiomyocytes. The endothelial nitric oxide synthase (eNOS) mRNA expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction. The activity of nitric oxide synthase (NOS) and NO content in cardiomyocytes were measured. The current results showed that U Ⅱ inhibited eNOS mRNA expression, the NOS activity and the NO production of cardiomyocytes. U Ⅱ (0. 1 μmol/L) inhibited the NOS activity and the NO production in cardiomyocytes in a time-dependent manner. These results suggest that the cardiovascular effect of U Ⅱ might be partially associated with NO production in cultured neonatal rat cardiomyocytes.
作者 李玲 袁文俊 潘秀颉 王伟忠 邱景伟 唐朝枢 唐朝枢
机构地区 第二军医大学基础部生理学教研室 北京大学第一医院心血管病研究所 北京大学医学部生理系
出处 《生理学报》 CAS CSCD 北大核心 2002年第4期307-310,共4页 Acta Physiologica Sinica
基金 This work was supported by the Major State Basic Research Development Program of People's Republic of China ( No. G2000056905) and the National Natural Science Fundation of China (No. 30070306).
关键词 尾加压素 新生大鼠 心肌细胞 一氧化氮合成 尿紧张素类 内皮型一氧化氮合酶 逆转录-多聚酶链反应 urotensins nitric oxide endothelial nitric oxide synthase cardiomyocytes reverse transcription-polymerase chain reaction
分类号 R331 [医药卫生—人体生理学]
  • 相关文献

参考文献10

  • 1[1]Coulouarn Y, Lihrman I, Jegou S, Anouar Y, Tostivint H, Beauvillain JC, Conlon JM, Bern HA, Vaudry H. Cloning of cDNA encoding the urotensinⅡprecursor in frog and human reveals intense expression of the urotensinⅡgene in motoneurons of the spinal cord. Proc Natl Acad Sci USA, 1998, 95:15803～15808.
  • 2[2]Ames RS, Sarau HM, Chambers JK, Willette RN, Aiyar NV, Romanic AN, Louden CS, Foley JJ, Sauermelch CF, Coatney RW, Ao Z, Disa J, Holmes SD, Stadel JM, Martin JD, Liu WS, Glover GI, Wilson S, Mcnulty DE, Ellis CE, Elshouragy NA, Shabon U, Trill JJ, Hay DWP, Ohlstein EH, Bergsma DJ, Douglas SA. Human urotensin-Ⅱis a potent vasoconstrictor and agonist for the orphan receptor GPR14. Nature, 1999, 401(6750):282～286.
  • 3[3]Russell FD, Molenaar P, O′Brien DM. Cardiostimulant effects of urotensin-Ⅱin human heart in vitro. Br J Pharmacol, 2001,132:5～9.
  • 4[4]Douglas SA,Ohlstein EH.Human urotensin-Ⅱ,the most potent mammalian vasoconstrictor identified to date, as a therapeutic target for the management of cardiovascular disease. Trends Cardiovasc Med, 2000, 10:229～237.
  • 5[5]Kumar A, Brar R, Wang P, Dee L, Skorupa G, Khadour F, Schulz R, Parrillo JE. Role of nitric oxide and cGMP in human septic serum-induced depression of cardiac myocyte contractility. Am J Physiol, 1999,276(1 Pt 2):R265～R276.
  • 6[6]Maclean MR, Alexander D, Seirrar A, Gallagher M, Douglas SA, Ohlstein EH, Morecroft I, Pollard K. Contractile responses to human urotensin-Ⅱin rat and human pulmonary arteries: effect of endothelial factors and chronic hypoxia in the rat. Br J Pharmacol, 2000,130:201～204.
  • 7[7]Gray GA, Jones MR, Sharif I. Human urotensinⅡ increases coronary perfusion pressure in the isolated rat heart: potentiation by nitric oxide synthase and cyclooxygenase inhibition. Life Sci, 2001,69:175～180.
  • 8[8]Katano Y, Ishihata A, Aita T, Ogaki T, Horie T. Vasodilator effect of urotensinⅡ, one of the most potent vasoconstricting factors, on rat coronary arteries. Eur J Pharmacol, 2000,402(1-2):R5～R7.
  • 9[9]Douglas SA, Sulpizio AC, Piercy V, Sarau HM, Ames RS, Aiyar NV, Ohlstein EH, Willette RN. Differential vasoconstrictor activity of human urotensin-Ⅱ in vascular tissue isolated from the rat, mouse, dog, pig, marmoset and cynomolgus monkey. Br J Pharmacol, 2000,131:1262～1274.
  • 10[10]Finkel MS, Oddis CV, Jacob TD, Watkins SC, Hattler BG, Simmons RL. Negative inotropic effects of cytokines on the heart mediated by nitric oxide. Science, 1992,257:387～389.

同被引文献101

  • 1LingLI,Wen-junYUAN,Ding-fengSU.Effects of rat urotensin Ⅱ on coronary flow and myocardial eNOS protein expression in isolated rat heart[J].Acta Pharmacologica Sinica,2004,25(11):1444-1449. 被引量：4
  • 2许丹,刘昌慧.尾加压素Ⅱ对人脐静脉内皮细胞iNOS表达的影响[J].中国应用生理学杂志,2006,22(3):288-291. 被引量：3
  • 3Pearson D, Shively JE, Clark BR, Geschwind, II, Barkley M, Nishioka RS, Bern HA. Urotensin II: a somatostatin-like peptide in the caudal neurosecretory system of fishes. Proc Natl Acad Sci U S A 1980; 77 (8): 5021-5024.
  • 4Ames RS, Sarau HM, Chambers JK, Willette RN, Aiyar NV, Romanic AM, Louden CS, Foley JJ, Sauermelch CF, Coatney RW, Ao Z, Disa J, Holmes SD, Stadel JM, Martin JD, Liu WS, Glover GI, Wilson S, McNulty DE, Ellis CE, Elshourbagy NA, Shabon U, Trill JJ, Hay DW, Ohlstein EH, Bergsma DJ, Douglas SA. Human urotensin-I1 is a potent vasoconstrictor and agonist for the orphan receptor GPR14. Nature 1999; 401 (6750): 282-286.
  • 5Douglas SA, Tayara L, Ohlstein EH, Halawa N, Giaid A. Congestive heart failure and expression of myocardial urotensin II. Lancet 2002; 359 (9322): 1990-1997.
  • 6Dschietzig T, Bartsch C, Pregla R, Zurbrugg HR, Armbruster FP, Richter C, Laule M, Romeyke E, Neubert C, Voelter W, Baumann G, Stangl K. Plasma levels and cardiovascular gene expression of urotensin-II in human heart failure. Regul Pept 2002; 110 (1): 33-38.
  • 7Douglas SA, Ashton DJ, Sauermelch CF, Coatney RW, Ohlstein DH, Ruffolo MR, Ohlstein EH, Aiyar NV, Willette RN. Human urotensin-II is a potent vasoactive peptide: pharmacological characterization in the rat, mouse, dog and primate. J Cardiovasc Pharmacol 2000; 36 (5 Suppl 1): S163-S166.
  • 8Coulouarn Y, Fernex C, Jegou S, Henderson CE, Vaudry H, Lihrmann I. Specific expression of the urotensin II gene in sacral motoneurons of developing rat spinal cord. Mech Dev 2001; 101 (1-2): 187-190.
  • 9Filipeanu CM, Brailoiu E, Le Dun S, Dun NJ. Urotensin-II regulates intracellular calcium in dissociated rat spinal cord neurons. J Neurochem 2002; 83 (4): 879-884.
  • 10Zhang AY, Chen YF, Zhang DX, Yi FX, Qi J, Andrade-Gordon P, de Garavilla L, Li PL, Zou AP. Urotensin II is a nitric oxide-dependent vasodilator and natriuretic peptide in the rat kidney. Am J Physiol Renal Physiol 2003; 285 (4): F792- F798.

引证文献7

二级引证文献21

生理学报
2002年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部