摘要
采用暂时性脑缺血再灌注大鼠模型,及H＆E、TUNEL细胞染色等实验技术,观察电针或碱性成纤维生长因子,以及两者合用对缺血性神经细胞死亡的影响。实验结果表明,电针与碱性成纤维生长因子合用与单纯使用电针或碱性成纤维生长因子相比,可明显减少暂时性脑缺血再灌注后神经细胞坏死和凋亡。提示碱性成纤维生长因子与电针可具有互补或加强的神经保护作用。两者合用具有一定的临床实际价值。
Transient cerebral ischemia/reperfusion rat model was adopted, and the method of H&E staining, TUNEL staining (TdT-mediated dUTP Nick End Labeling) were used to observe the effect of electroacupuncture (EA) and basic fibroblast growth factor (bFGF) on neuronal death. The results e-vinced that the combination of EA and bFGF could evidently reduce the neuronal death, including both necrosis and apoptosis, compared with EA or bFGF application alone. It is suggested that bFGF and EA can complement each other and enhance the protective effect following cerebral ischemia.
出处
《生理学报》
CAS
CSCD
北大核心
2002年第4期321-324,共4页
Acta Physiologica Sinica
基金
This work was supported by the National Natural Science Foundation of China ( No. 39730510) and a grant from the Traditional Chinese Medicine Programs of Shanghai Medicine Bureau ( Xing Lin Program) ( No. 2000-3 ).
关键词
电针
碱性成纤维生长因子
BFGF
脑缺血
神经保护作用
cerebral ischemia/reperfusion
electroacupuncture (EA)
basic fibroblast growth factor (bFGF)
neuro-protective effect
