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药用植物川东獐牙菜的组织培养 被引量:10

Tissue culture of medical plant Swertia davidii Franch
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摘要 针对川东獐牙菜野生资源受到严重破坏的情况 ,系统地探讨了通过组织培养为手段进行人工繁殖的方法 ,旨在为川东獐牙菜的保护提供坚实的理论依据。研究结果表明 :在所有的实验方案中 ,幼茎和老叶是理想的外植体材料。对叶片来说 ,较适宜的诱导愈伤组织的激素组合是 Zt1.0 m g/L +NAA 0 .0 5m g/L +IBA0 .0 5mg/L、BA 0 .5m g/L+2 ,4 - D0 .5mg/L或 BA 0 .2 m g/L+2 ,4 - D0 .2 mg/L+IBA0 .1m g/L ,对茎段来说 ,较适宜的诱导愈伤组织的激素组合是 BA0 .0 5m g/L+kt0 .0 5mg/L+IBA0 .0 5m g/L;诱导不定芽的适宜激素组合是 BA2 .0 mg/L+N AA0 .0 5mg/L或 BA 2 .0 mg/L+IBA 0 .1m g/L ;而根的诱导则是 MS+BA 0 .0 5mg/L+kt1.0 mg/L+N AA0 .1m g/L或 MS+BA1.0 m g/L+kt0 .3mg/L +IA A0 .5mg/L培养基上进行。 In order to protect the natural resources of Swertia davidii Franch. which has been destroyed seriously, the method of artificial propagation by way of tissue culture has been systematically researched. The results showed that the mature stems and the immature leaves are the best material explants in speeding propagation in all of the experiments. As for leaves, the suitable phytohormone compositions to induct callus is Zt1.0 mg/L+NAA0.05 mg/L?BA0.5 mg/L+2,4-D0.5 mg/L+IBA0.05 mg/L or BA0.2 mg/L+2,4-D0.2 mg/L+IBA0.1 mg/L. As for stems, the suitable phytohormone compositions to induct callus is BA0.05 mg/L+kt0.05 mg/L+IBA0.05 mg/L. The suitable phytohormone compositions to induct adventitious buds is BA2.0 mg/L+NAA0.05 mg/L or BA2.0 mg/L+IBA0.1 mg/L, and that of induct roots is MS+BA0.05 mg/L+kt1.0 mg/L+NAA0.1 mg/L or MS+BA1.0 mg/L+kt0.3 mg/L+IAA0.5 mg/L.
机构地区 吉首大学生物系
出处 《广西植物》 CAS CSCD 北大核心 2002年第5期433-436,432,共5页 Guihaia
基金 湖南省教育厅资助项目 (编号 :0 0 C191)
关键词 药用植物 川东獐牙菜 组织培养 愈伤组织 芽丛 生根 Swertia davidii Franch tissue culture callus adventitious bud rooting
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