激素对樱桃番茄两种外植体诱导再生植株的影响

Effects of phytohormones on regeneration from two explants in Lycopersicum esculentum

以 MS为基本培养基 ,附加不同浓度的 6-BA、IAA和 NAA培养樱桃番茄两种外植体以诱导再生植株。结果表明 :含 NAA的 MS+6-BA2 mg/L(单位下同 ) +NAA0 .2培养基诱导的叶片愈伤组织 ,经继续培养无芽的分化 ,含 IAA的 MS+6-BA2 +IAA0 .2培养基诱导的愈伤组织 ,经继续培养诱导芽的分化 ;含 NAA或IAA的 MS+6-BA2 +NAA0 .2和 MS+6-BA2 +IAA0 .2培养基利于下胚轴愈伤组织的诱导 ,而不含生长素的 MS+6-BA1 .0培养基可直接诱导芽的分化。

Two explants from Lycopersicum esculentum were cultured on MS medium supplemented with 6-BA,IAA and NAA of different density to induce regeneration plants. The results showed that the callus from leaf which has been induced on medium MS+6-BA2 mg/L(Unit is the same as follows)+NAA0.2 is not differentiated into bud by subculture, and on medium MS+6-BA2+IAA0.2 it can be differentiated into bud by subculture. It is favorable to induce callus of hypocotyl on medium MS+6-BA2.0+NAA0.2 and MS+6-BA2.0+IAA0.2, and bud may be directly induced with hypocotyl on medium MS+6-BA1.0.