活体动物全细胞记录技术及其应用

IN VIVO WHOLE-CELL RECORDING TECHNIQUE AND ITS APPLICATION

活体动物全细胞记录技术不仅可以用于研究感觉系统对自然刺激(如视觉系统的光刺激、听觉系统的声音刺激等)反应的特性和规律,还可以较准确地记录细胞的突触电位(包括阈下反应),实现EPSP和IPSP的相对分离,并实现活体细胞胞内灌流,从而进一步研究感觉信息的处理机制。本文较为详细地介绍了在活体动物上进行全细胞记录的方法,包括一些技术细节和关键仪器设备的选取原则,举例说明了该技术在视觉系统研究和体感系统研究中的应用,并讨论了这一方法在神经科学中的应用前景。

In vivo whole-cell recording technique can be used not on ly to study the properties of re-sponse to natural stimulus in sensory system, such as light for visual system and sound for auditory sys-t em, but also to study the mechanism of sensory information processing, because a cell's synaptic poten-tial, including subthreshold response, can be recorded accurately, and the cell's EPSPs and IPSPs can be s eparated relatively. This technique, including how to choose some major instruments, is described in de-tail. The results illustrate its appl ication in visual system and somatosensory system. The prospect of thi s technique in neuroscience is also discussed.