长柔毛野豌豆编码甘油-3-磷酸酰基转移酶基因的克隆及序列分析

Cloning and Nucleotide Sequence of cDNA for the Glycerol-3-phosphate Acyltsansferase from Vicia villosa

甘油 - 3-磷酸酰基转移酶 (GPAT)基因与植物抗冷性密切相关。克隆到的长柔毛野豌豆 (Viciavillosa)GPAT基因的编码区完整的cDNA片段长 1377bp ,编码 4 5 8个氨基酸残基 ,与蚕豆 (Viciafaba)和豌豆 (Pissumsativum)比较 ,其核苷酸序列的同源性分别为 94 1%和93 3% ,氨基酸序列的同源性分别为 96 9%和 98 0 %。

Glycerol 3 phosphate acyltransferase(GPAT) gene is closely relative to the chilling resistance of plants. We isolated the cDNA encoding the GPAT from Vicia villosa cotyledons.The cDNA fragement includes the whole coding region,which contains 1377 base pairs and codes 458 amino acids. Comparing to Vicia faba and Pissum sativum ,the cDNA sequence and the deduced amino acid sequence of Vicia villosa GPAT gene have 94 1% and 93 3%,96 9% and 98 0% homology respectively.